Hotspot

trnN-GUU-ycf1

SSC · 113134-113462 · 329 bp

Back to Menispermum

Pi 0.0426

MarkerSeek score 64.20

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: SSC
length_bp: 329
variable_sites: 14
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	TCAAAGCGAGCCCTTTCATT	TGATCAAGAAGGAGGGTGGT	45.0	50.0	58.1	58.0	1093-1140 bp	1.0	83.1
trnN-GUU-ycf1_p2	ATGGAACTTGGTTGTGGGTC	TGATCAAGAAGGAGGGTGGT	50.0	50.0	58.0	58.0	1598-1644 bp	1.0	82.6
trnN-GUU-ycf1_p3	GGAACTTGGTTGTGGGTCTT	TGATCAAGAAGGAGGGTGGT	50.0	50.0	57.9	58.0	1596-1642 bp	1.0	82.1
trnN-GUU-ycf1_p4	ATGGAACTTGGTTGTGGGTC	AGAAACCTCCCTGTATCCCC	50.0	55.0	58.0	58.1	1700-1746 bp	1.0	81.9
trnN-GUU-ycf1_p5	TGTTTTACTCCCCGCAACTC	TGATCAAGAAGGAGGGTGGT	50.0	50.0	58.1	58.0	1470-1517 bp	1.0	81.9