Hotspot

rpoB-trnC-GCA

LSC · 28228-29531 · 1304 bp

Back to Menispermum

Pi 0.0507

MarkerSeek score 86.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1304
variable_sites: 66
pi: 0.0507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GAACCCACGAAATCCCTCAA	TGTTGTTGTTGATCAGGCGA	50.0	45.0	57.8	58.0	1467-1490 bp	1.0	81.8
rpoB-trnC-GCA_p2	GAACCCACGAAATCCCTCAA	TTGTTGTTGATCAGGCGACA	50.0	45.0	57.8	58.0	1465-1488 bp	1.0	81.8
rpoB-trnC-GCA_p3	GAACCCACGAAATCCCTCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1444-1467 bp	1.0	81.5
rpoB-trnC-GCA_p4	TCAAGCCCCGATCAATGAAC	TGTTGTTGTTGATCAGGCGA	50.0	45.0	58.2	58.0	1483-1506 bp	1.0	81.3
rpoB-trnC-GCA_p5	TCAAGCCCCGATCAATGAAC	TTGTTGTTGATCAGGCGACA	50.0	45.0	58.2	58.0	1481-1504 bp	1.0	81.3