Hotspot

atpF-atpH

LSC · 14370-14619 · 250 bp

Back to Menispermum

Pi 0.0486

MarkerSeek score 81.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 250
variable_sites: 12
pi: 0.0486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGATTGGTCGTGGCATTAG	50.0	50.0	58.0	58.0	338-340 bp	1.0	82.9
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGATTGGTCGTGGCATTAG	50.0	50.0	57.9	58.0	366-368 bp	1.0	82.0
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	TTTATGGATTGGTCGTGGCA	50.0	45.0	58.0	57.5	342-344 bp	1.0	79.2
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	CCAGAGGCAGAGGGAAAAAT	50.0	50.0	58.0	57.5	409-411 bp	1.0	78.4
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	TTTATGGATTGGTCGTGGCA	50.0	45.0	57.9	57.5	370-372 bp	1.0	78.3