Hotspot

trnH-GUG-psbA

LSC · 158-738 · 581 bp

Back to Menispermum

Pi 0.0197

MarkerSeek score 70.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 11
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TGGGCTGATATCATCAACCG	50.0	50.0	57.9	57.5	707-724 bp	1.0	78.2
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	GGGCTGATATCATCAACCGT	50.0	50.0	57.9	57.4	706-723 bp	1.0	78.2
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	TGGGCTGATATCATCAACCG	50.0	50.0	58.2	57.5	708-725 bp	1.0	78.0
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	GGGCTGATATCATCAACCGT	50.0	50.0	58.2	57.4	707-724 bp	1.0	78.0
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	TGGGCTGATATCATCAACCG	50.0	50.0	57.7	57.5	700-717 bp	1.0	76.7