primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	TGGGCTGATATCATCAACCG	20	20	50.0	50.0	57.9	57.5	0.0	5.7	0.683853	158	738	707-724	1.000000	78.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CTGCCTTAATCCACTTGGCT	GGGCTGATATCATCAACCGT	20	20	50.0	50.0	57.9	57.4	0.0	5.7	0.685619	158	738	706-723	1.000000	78.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACTGCCTTAATCCACTTGGC	TGGGCTGATATCATCAACCG	20	20	50.0	50.0	58.2	57.5	0.0	5.7	0.709640	158	738	708-725	1.000000	78.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACTGCCTTAATCCACTTGGC	GGGCTGATATCATCAACCGT	20	20	50.0	50.0	58.2	57.4	0.0	5.7	0.711407	158	738	707-724	1.000000	78.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTTGGCTACATCCG	TGGGCTGATATCATCAACCG	20	20	50.0	50.0	57.7	57.5	0.0	5.7	0.881127	158	738	700-717	1.000000	76.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTACCAACGGTATGGACGA	TCCTTGAAAAAGGTGCCCAA	20	20	50.0	45.0	57.9	57.8	10.6	4.5	0.294178	4862	5806	1112-1157	1.000000	81.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCTACCAACGGTATGGACGA	TTCCTTGAAAAAGGTGCCCA	20	20	50.0	45.0	57.9	57.8	10.6	4.5	0.294178	4862	5806	1113-1158	1.000000	81.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TCCTTGAAAAAGGTGCCCAA	20	20	50.0	45.0	58.2	57.8	20.3	4.5	0.335327	4862	5806	1047-1092	1.000000	81.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TCCTTGAAAAAGGTGCCCAA	20	20	50.0	45.0	58.2	57.8	20.3	4.5	0.335327	4862	5806	1046-1091	1.000000	81.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGTGCCCA	20	20	50.0	45.0	58.2	57.8	20.3	4.5	0.335327	4862	5806	1047-1092	1.000000	81.7
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	AATCCTGGGCGTGAGGAATA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.5	58.0	3.0	0.0	0.497443	9067	9211	239-239	1.000000	77.7
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATCCTGGGCGTGAGGAATAA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.5	58.0	3.0	0.0	0.497443	9067	9211	238-238	1.000000	77.7
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	TAATCCTGGGCGTGAGGAAT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.5	58.0	3.0	0.0	0.497443	9067	9211	240-240	1.000000	77.7
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	AATCCTGGGCGTGAGGAATA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.5	58.0	3.0	0.0	0.498831	9067	9211	240-240	1.000000	77.7
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATCCTGGGCGTGAGGAATAA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.5	58.0	3.0	0.0	0.498831	9067	9211	239-239	1.000000	77.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.976483	9300	10322	1103-1135	1.000000	76.9
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.976483	9300	10322	1102-1134	1.000000	76.9
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AATCCGACGCTTTAGTCCAC	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.977871	9300	10322	1102-1134	1.000000	76.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.0	57.0	0.0	0.0	0.977871	9300	10322	1101-1133	1.000000	76.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.2	57.0	0.0	0.0	1.123249	9300	10322	1156-1188	1.000000	75.5
atpF-atpH_p1	atpF-atpH	1	CAGTGACCCAAGGAAACGAA	TGGATTGGTCGTGGCATTAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075164	14370	14619	338-340	1.000000	82.9
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	TGGATTGGTCGTGGCATTAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.155271	14370	14619	366-368	1.000000	82.0
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAACGAA	TTTATGGATTGGTCGTGGCA	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.536851	14370	14619	342-344	1.000000	79.2
atpF-atpH_p4	atpF-atpH	4	CAGTGACCCAAGGAAACGAA	CCAGAGGCAGAGGGAAAAAT	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.559753	14370	14619	409-411	1.000000	78.4
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	TTTATGGATTGGTCGTGGCA	20	20	50.0	45.0	57.9	57.5	0.0	0.0	0.616957	14370	14619	370-372	1.000000	78.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GAACCCACGAAATCCCTCAA	TGTTGTTGTTGATCAGGCGA	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.233785	28228	29531	1467-1490	1.000000	81.8
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GAACCCACGAAATCCCTCAA	TTGTTGTTGATCAGGCGACA	20	20	50.0	45.0	57.8	58.0	0.0	14.7	0.233785	28228	29531	1465-1488	1.000000	81.8
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GAACCCACGAAATCCCTCAA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.276329	28228	29531	1444-1467	1.000000	81.5
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCAAGCCCCGATCAATGAAC	TGTTGTTGTTGATCAGGCGA	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.289390	28228	29531	1483-1506	1.000000	81.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCAAGCCCCGATCAATGAAC	TTGTTGTTGATCAGGCGACA	20	20	50.0	45.0	58.2	58.0	0.0	14.7	0.289390	28228	29531	1481-1504	1.000000	81.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	50377	51419	1057-1093	1.000000	82.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	50377	51419	1065-1101	1.000000	81.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	50377	51419	1089-1125	1.000000	81.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	50377	51419	1097-1133	1.000000	81.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	50377	51419	1080-1116	1.000000	80.6
accD-psaI_p1	accD-psaI	1	AGTTATTTCAGCTGCATGGGT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.9	58.0	9.5	26.9	1.118636	63059	63804	860-885	1.000000	73.1
accD-psaI_p2	accD-psaI	2	AGTTATTTCAGCTGCATGGGT	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	57.9	57.9	9.5	26.8	1.195170	63059	63804	866-891	1.000000	72.4
accD-psaI_p3	accD-psaI	3	TTTCAGCTGCATGGGTTTTT	CCATTGCAATTGCCGGAAAT	20	20	40.0	45.0	56.7	58.0	9.5	26.9	1.324723	63059	63804	855-880	1.000000	71.4
accD-psaI_p4	accD-psaI	4	TTTCAGCTGCATGGGTTTTT	AAGAAGCCATTGCAATTGCC	20	20	40.0	45.0	56.7	57.9	9.5	26.8	1.401257	63059	63804	861-886	1.000000	70.8
accD-psaI_p5	accD-psaI	5	AGTTATTTCAGCTGCATGGGT	AGGCACCAAAATAGAGGGGA	21	20	42.9	50.0	57.9	58.3	9.5	0.0	1.410404	63059	63804	826-851	1.000000	70.8
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	57.9	58.1	8.4	0.0	0.158370	64898	65619	818-828	1.000000	81.7
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	GAGATCCACCAGGGCAAAAA	20	20	50.0	50.0	58.1	58.1	8.4	0.0	0.194446	64898	65619	820-830	1.000000	81.5
ycf4-cemA_p3	ycf4-cemA	3	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	64898	65619	845-855	1.000000	80.9
ycf4-cemA_p4	ycf4-cemA	4	AAAGCTGCTGAATTGGCCTA	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	64898	65619	843-853	1.000000	80.5
ycf4-cemA_p5	ycf4-cemA	5	TGCGCGTACCAATTGAAGTA	GAGAGAGAGATCCACCAGGG	20	20	45.0	60.0	57.9	58.3	8.4	0.0	0.378315	64898	65619	824-834	1.000000	80.0
rrn23_p1	rrn23	1	TCCTTCCATTTAGCAGCACC	AAACTTCTGTCGCTCCATCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.229375	108747	111550	2943-2943	1.000000	79.2
rrn23_p2	rrn23	2	CCTTCCATTTAGCAGCACCT	AAACTTCTGTCGCTCCATCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.235394	108747	111550	2942-2942	1.000000	79.1
rrn23_p3	rrn23	3	CGATTCGGCAGTTCGATCTA	AAACTTCTGTCGCTCCATCC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.501622	108747	111550	2992-2992	1.000000	77.0
rrn23_p4	rrn23	4	AGCCTTAAAGTTAACGGCGA	AAACTTCTGTCGCTCCATCC	20	20	45.0	50.0	57.5	57.9	16.6	0.0	0.561362	108747	111550	2911-2911	1.000000	76.5
rrn23_p5	rrn23	5	GCACCTTAGGATGGCATAGC	AAACTTCTGTCGCTCCATCC	20	20	55.0	50.0	58.5	57.9	4.8	0.0	0.572475	108747	111550	2928-2928	1.000000	76.4
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	TCAAAGCGAGCCCTTTCATT	TGATCAAGAAGGAGGGTGGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.135093	113134	113462	1093-1140	1.000000	83.1
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	ATGGAACTTGGTTGTGGGTC	TGATCAAGAAGGAGGGTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049416	113134	113462	1598-1644	1.000000	82.6
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GGAACTTGGTTGTGGGTCTT	TGATCAAGAAGGAGGGTGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.112158	113134	113462	1596-1642	1.000000	82.1
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	ATGGAACTTGGTTGTGGGTC	AGAAACCTCCCTGTATCCCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.109924	113134	113462	1700-1746	1.000000	81.9
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	TGTTTTACTCCCCGCAACTC	TGATCAAGAAGGAGGGTGGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.157906	113134	113462	1470-1517	1.000000	81.9
ycf1-ndhF_p1	ycf1-ndhF	1	TCGTAGTAGCTCCGTTCCAT	TGATCAAGAAGGAGGGTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.093237	113485	113578	877-924	1.000000	84.5
ycf1-ndhF_p2	ycf1-ndhF	2	GAGCTACTGAGGAACAACGG	TGATCAAGAAGGAGGGTGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.047124	113485	113578	984-1031	1.000000	84.3
ycf1-ndhF_p3	ycf1-ndhF	3	GCTTCCTTCGTAACTCTCGG	TGATCAAGAAGGAGGGTGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.134105	113485	113578	904-951	1.000000	84.0
ycf1-ndhF_p4	ycf1-ndhF	4	GAGCTACTGAGGAACAACGG	AGAAACCTCCCTGTATCCCC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.107632	113485	113578	1086-1133	1.000000	83.4
ycf1-ndhF_p5	ycf1-ndhF	5	TCAAAGCGAGCCCTTTCATT	TGATCAAGAAGGAGGGTGGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.135093	113485	113578	1093-1140	1.000000	83.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CAAAGCGTTTTGGGCAGTAC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	16.1	0.0	0.358208	117383	118257	1023-1049	1.000000	79.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CAAAGCGTTTTGGGCAGTAC	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.2	57.8	16.1	0.0	0.418935	117383	118257	1016-1042	1.000000	79.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CAAAGCGTTTTGGGCAGTAC	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.2	57.8	16.1	0.0	0.430226	117383	118257	1022-1048	1.000000	79.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CAAAGCGTTTTGGGCAGTAC	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.2	57.8	16.1	0.0	0.430226	117383	118257	1021-1047	1.000000	79.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAGCGTTTTGGGCAGTACT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.436714	117383	118257	1022-1048	1.000000	79.3
ccsA-ndhD_p1	ccsA-ndhD	1	TGCAATTTTGGCTTCTATGGG	TCCGGATTTTGTTTTCTCCCT	21	21	42.9	42.9	57.1	57.5	0.0	0.0	3.410414	119396	119717	498-519	1.000000	54.1
ccsA-ndhD_p2	ccsA-ndhD	2	GCAATTTTGGCTTCTATGGGA	TCCGGATTTTGTTTTCTCCCT	21	21	42.9	42.9	56.8	57.5	0.0	0.0	3.688917	119396	119717	497-518	1.000000	51.9
ccsA-ndhD_p3	ccsA-ndhD	3	CTGCAATTTTGGCTTCTATGGG	TCCGGATTTTGTTTTCTCCCT	22	21	45.5	42.9	58.2	57.5	0.0	0.0	3.730055	119396	119717	499-520	1.000000	51.6
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAATTTTGGCTTCTATGGG	ATCCGGATTTTGTTTTCTCCCT	21	22	42.9	40.9	57.1	58.0	0.0	12.4	3.939049	119396	119717	499-520	1.000000	49.9
ccsA-ndhD_p5	ccsA-ndhD	5	TGCAATTTTGGCTTCTATGGG	TCCGGATTTTGTTTTCTCCC	21	20	42.9	45.0	57.1	55.9	0.0	0.0	3.961854	119396	119717	498-519	1.000000	49.7
ndhA_p1	ndhA	1	ACCCAATTACCATAGGAACCA	TGGGAGAAGTTGATCGGTGA	21	20	42.9	50.0	56.3	58.4	0.0	0.0	3.063579	124251	126548	2415-2424	1.000000	57.9
ndhA_p2	ndhA	2	AACCCAATTACCATAGGAACCA	TGGGAGAAGTTGATCGGTGA	22	20	40.9	50.0	57.0	58.4	0.0	0.0	3.317884	124251	126548	2416-2425	1.000000	55.8
ndhA_p3	ndhA	3	ACCCAATTACCATAGGAACCA	ATGGGAGAAGTTGATCGGTG	21	20	42.9	50.0	56.3	57.3	0.0	0.0	3.402156	124251	126548	2416-2425	1.000000	55.2
ndhA_p4	ndhA	4	ACCCAATTACCATAGGAACCA	GGGAGAAGTTGATCGGTGAA	21	20	42.9	50.0	56.3	57.2	0.0	0.0	3.465837	124251	126548	2414-2423	1.000000	54.7
ndhA_p5	ndhA	5	GAACCCAATTACCATAGGAACCA	TGGGAGAAGTTGATCGGTGA	23	20	43.5	50.0	58.1	58.4	0.0	0.0	3.497906	124251	126548	2417-2426	1.000000	54.4
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	GTAGAACCCAACCACAACGA	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	57.8	58.2	0.0	15.0	0.400234	134143	134471	469-509	1.000000	78.4
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TAGAACCCAACCACAACGAC	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	57.8	58.2	0.0	15.0	0.400234	134143	134471	468-508	1.000000	78.4
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	AGTAGAACCCAACCACAACG	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	57.8	58.2	0.0	15.0	0.406475	134143	134471	470-510	1.000000	78.4
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GTAGAACCCAACCACAACGA	AAAGCGAGCCCTTTCATTCT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.450178	134143	134471	527-567	1.000000	77.9
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	TAGAACCCAACCACAACGAC	AAAGCGAGCCCTTTCATTCT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.450178	134143	134471	526-566	1.000000	77.9