Hotspot

ycf1

SSC · 126721-132315 · 5595 bp

Back to Memecylon

Pi 0.0059

MarkerSeek score 53.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5595
variable_sites: 33
pi: 0.0059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCTTGAAAAATAGAATGGTTTTCA	TTGGGGAATGGAAACTGACC	30.8	50.0	57.7	57.7	220 bp	1.0	42.2
ycf1_p2	TCCCTTGAAAAATAGAATGGT	TTGGGGAATGGAAACTGACC	33.3	50.0	52.7	57.7	220 bp	1.0	42.2
ycf1_p3	TCCCTTGAAAAATAGAATGGTT	TTGGGGAATGGAAACTGACC	31.8	50.0	53.6	57.7	220 bp	1.0	42.2
ycf1_p4	TTCCCTTGAAAAATAGAATGGT	TTGGGGAATGGAAACTGACC	31.8	50.0	53.6	57.7	221 bp	1.0	42.2
ycf1_p5	TCCCTTGAAAAATAGAATGGTTTTCA	TGGGGAATGGAAACTGACCT	30.8	50.0	57.7	58.5	219 bp	1.0	42.2