Hotspot

clpP

LSC · 72132-74248 · 2117 bp

Back to Memecylon

Pi 0.00426

MarkerSeek score 60.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2117
variable_sites: 9
pi: 0.00426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCTTAAACTTACCCTACCCC	AAACGCATTGTTACGCTTCC	47.6	45.0	57.0	57.7	2230-2232 bp	1.0	61.7
clpP_p2	ACTTACCCTACCCCTACTCT	AAACGCATTGTTACGCTTCC	50.0	45.0	55.7	57.7	2223-2225 bp	1.0	59.7
clpP_p3	GCTTAAACTTACCCTACCCCT	AAACGCATTGTTACGCTTCC	47.6	45.0	56.7	57.7	2229-2231 bp	1.0	59.4
clpP_p4	TTGCTTAAACTTACCCTACCCC	AAACGCATTGTTACGCTTCC	45.5	45.0	57.7	57.7	2231-2233 bp	1.0	59.3
clpP_p5	AACTTACCCTACCCCTACTCT	AAACGCATTGTTACGCTTCC	47.6	45.0	56.6	57.7	2224-2226 bp	1.0	58.2