Hotspot

atpF

LSC · 11982-13326 · 1345 bp

Back to Memecylon

Pi 0.00446

MarkerSeek score 54.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1345
variable_sites: 6
pi: 0.00446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATGGTTACC	AGTGAAACAAAGGAGATTGT	50.0	35.0	56.9	52.6	1439 bp	1.0	40.3
atpF_p2	CGTCGGCTTTAATGGTTACC	AAGTGAAACAAAGGAGATTGT	50.0	33.3	56.9	53.5	1440 bp	1.0	40.3
atpF_p3	CGTCGGCTTTAATGGTTACC	TCTGTAAAAGTGAAACAAAGGAGA	50.0	33.3	56.9	56.4	1447 bp	1.0	40.3
atpF_p4	CGTCGGCTTTAATGGTTACC	AAAGTGAAACAAAGGAGATTGT	50.0	31.8	56.9	54.4	1441 bp	1.0	40.3
atpF_p5	TCGTCGGCTTTAATGGTTACC	AGTGAAACAAAGGAGATTGT	47.6	35.0	58.4	52.6	1440 bp	1.0	40.3