Hotspot

rpl14-rps3

LSC · 83559-85292 · 1734 bp

Back to Memecylon

Pi 0.00577

MarkerSeek score 61.60

Primer available yes

Label rpl14-rps3

Feature Metadata

label_name: rpl14-rps3
feature_type: igs
region: LSC
length_bp: 1734
variable_sites: 10
pi: 0.00577
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps3_p1	CGCGACATTCAAATGGCTTT	CGATCTATGGGGTATTGGGC	45.0	55.0	58.0	57.6	1820 bp	1.0	77.1
rpl14-rps3_p2	CGCGACATTCAAATGGCTTT	CTCTACAAACCATTCGGGCT	45.0	50.0	58.0	57.6	1871 bp	1.0	77.1
rpl14-rps3_p3	ACACATCAATTCTCGAGCCC	CGATCTATGGGGTATTGGGC	50.0	55.0	58.0	57.6	1850 bp	1.0	77.0
rpl14-rps3_p4	ACACATCAATTCTCGAGCCC	CTCTACAAACCATTCGGGCT	50.0	50.0	58.0	57.6	1901 bp	1.0	77.0
rpl14-rps3_p5	CGCGACATTCAAATGGCTTT	CCTCTACAAACCATTCGGGC	45.0	55.0	58.0	58.6	1872 bp	1.0	75.4