Pi
0.00318
MarkerSeek score
61.30
Primer available
yes
Label
petA-psbJ
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| petA-psbJ_p1 | GGTCTTTTGTTCTTCTTGGCA | GGGTTGGGCTCATCTTTGTA | 42.9 | 50.0 | 56.9 | 57.5 | 1072-1073 bp | 1.0 | 60.1 |
| petA-psbJ_p2 | AGAAGCAGTTTGAGAAGGTTCA | GGGTTGGGCTCATCTTTGTA | 40.9 | 50.0 | 57.8 | 57.5 | 1014-1015 bp | 1.0 | 59.5 |
| petA-psbJ_p3 | GGTCTTTTGTTCTTCTTGGCA | ATGGTTCATACTCCGGGTTG | 42.9 | 50.0 | 56.9 | 57.3 | 1086-1087 bp | 1.0 | 58.5 |
| petA-psbJ_p4 | AGAAGCAGTTTGAGAAGGTTCA | ATGGTTCATACTCCGGGTTG | 40.9 | 50.0 | 57.8 | 57.3 | 1028-1029 bp | 1.0 | 57.8 |
| petA-psbJ_p5 | AGGTCTTTTGTTCTTCTTGGC | GGGTTGGGCTCATCTTTGTA | 42.9 | 50.0 | 56.6 | 57.5 | 1073-1074 bp | 1.0 | 57.8 |