Hotspot

rbcL-accD

LSC · 58482-59348 · 867 bp

Back to Memecylon

Pi 0.00964

MarkerSeek score 59.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 8
pi: 0.00964
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	CGGATCTTCACTTCCACGAG	55.0	55.0	57.7	58.1	1026-1063 bp	1.0	80.4
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	TTTCCCGTTAGACGACATCG	55.0	50.0	57.7	57.7	948-985 bp	1.0	79.3
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACATCGAATTAAACCGCCGT	55.0	45.0	57.7	58.3	934-971 bp	1.0	79.3
rbcL-accD_p4	GCTGCTGCTTGTGAAGTATG	CGGATCTTCACTTCCACGAG	50.0	55.0	57.5	58.1	1013-1050 bp	1.0	78.5
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GACATCGAATTAAACCGCCG	55.0	50.0	57.7	57.6	935-972 bp	1.0	78.5