Hotspot

atpB-rbcL

LSC · 56230-57053 · 824 bp

Back to Memecylon

Pi 0.00485

MarkerSeek score 61.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 4
pi: 0.00485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGAGCGATACGTCCTAGGTT	ACACCAGCTTTGAATCCAACA	50.0	42.9	58.0	58.3	948 bp	1.0	69.3
atpB-rbcL_p2	TTGAGCGATACGTCCTAGGT	ACACCAGCTTTGAATCCAACA	50.0	42.9	58.0	58.3	949 bp	1.0	69.3
atpB-rbcL_p3	TGAGCGATACGTCCTAGGTT	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.0	56.6	919 bp	1.0	69.0
atpB-rbcL_p4	TTGAGCGATACGTCCTAGGT	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.0	56.6	920 bp	1.0	69.0
atpB-rbcL_p5	TGAGCGATACGTCCTAGGTT	CACCAGCTTTGAATCCAACA	50.0	45.0	58.0	56.5	947 bp	1.0	68.2