Hotspot

atpH-atpI

LSC · 14137-15074 · 938 bp

Back to Memecylon

Pi 0.00441

MarkerSeek score 64.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 4
pi: 0.00441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.0	57.9	983-1014 bp	1.0	81.4
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.0	58.1	995-1026 bp	1.0	81.3
atpH-atpI_p3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1013-1044 bp	1.0	81.0
atpH-atpI_p4	GGCAGAAATCAGTGGGTTCA	TAGCCGCCGCTTATATAGGA	50.0	50.0	58.1	57.9	972-1003 bp	1.0	81.0
atpH-atpI_p5	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1025-1056 bp	1.0	80.9