Hotspot

atpF-atpH

LSC · 13327-13890 · 564 bp

Back to Memecylon

Pi 0.016

MarkerSeek score 65.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 9
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCCAAGGAAAGGAAAGAA	GTCTGGCTTTTATGGAGGCA	50.0	50.0	58.0	57.9	681-682 bp	1.0	80.1
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GTCTGGCTTTTATGGAGGCA	50.0	50.0	57.9	57.9	713-714 bp	1.0	79.1
atpF-atpH_p3	TTGGCCCAAGGAAAGGAAAG	GTCTGGCTTTTATGGAGGCA	50.0	50.0	58.3	57.9	683-684 bp	1.0	77.9
atpF-atpH_p4	GGCCCAAGGAAAGGAAAGAA	AGTCTGGCTTTTATGGAGGC	50.0	50.0	58.0	57.6	682-683 bp	1.0	77.7
atpF-atpH_p5	CATTGGCCCAAGGAAAGGAA	GTCTGGCTTTTATGGAGGCA	50.0	50.0	58.4	57.9	685-686 bp	1.0	77.3