Hotspot

rps16-trnQ-UUG

LSC · 5708-6766 · 1059 bp

Back to Memecylon

Pi 0.00756
MarkerSeek score 63.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1059
variable_sites
8
pi
0.00756
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTGCTACCACATCGTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1120-1121 bp 1.0 79.2
rps16-trnQ-UUG_p2 TTGCTTGCTACCACATCGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1121-1122 bp 1.0 79.2
rps16-trnQ-UUG_p3 ACGATCATGTCCGTCAAGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1146-1147 bp 1.0 77.8
rps16-trnQ-UUG_p4 GCTTGCTACCACATCGTTTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1119-1120 bp 1.0 75.8
rps16-trnQ-UUG_p5 TGCTTGCTACCACATCGTTT TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1124-1125 bp 1.0 74.4