Hotspot

ycf24

Genome · 128205-129668 · 1464 bp

Back to Membranoptera

Pi 0.0123

MarkerSeek score 52.70

Primer available yes

Label ycf24

Feature Metadata

label_name: ycf24
feature_type: gene
region: Genome
length_bp: 1464
variable_sites: 18
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf24_p1	CCCACCTGAAAATCCTTCGT	TCCCTTCTAAGCAGAGAAATCT	50.0	40.9	57.8	56.3	2291 bp	0.5	28.5
ycf24_p2	TGTGCTGTTGTTGAATTGCC	TCCCTTCTAAGCAGAGAAATCT	45.0	40.9	57.8	56.3	2545 bp	0.5	28.3
ycf24_p3	CCCACCTGAAAATCCTTCGT	TTCCCTTCTAAGCAGAGAAATCT	50.0	39.1	57.8	57.0	2292 bp	0.5	26.2
ycf24_p4	TGCTGTTGTTGAATTGCCTG	TCCCTTCTAAGCAGAGAAATCT	45.0	40.9	57.5	56.3	2543 bp	0.5	26.0
ycf24_p5	TGTGCTGTTGTTGAATTGCC	TTCCCTTCTAAGCAGAGAAATCT	45.0	39.1	57.8	57.0	2546 bp	0.5	26.0