Hotspot

orf382

Genome · 48577-49653 · 1077 bp

Back to Membranoptera

Pi 0.0158

MarkerSeek score 54.40

Primer available yes

Label orf382

Feature Metadata

label_name: orf382
feature_type: gene
region: Genome
length_bp: 1077
variable_sites: 17
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf382_p1	GAAGGACTTGAACCCTCGAC	GCCGATATTAGCGCAATCCT	55.0	50.0	57.9	58.3	1419 bp	0.5	57.2
orf382_p2	AGCTCCATGTTGACCCAAAG	GCCGATATTAGCGCAATCCT	50.0	50.0	58.1	58.3	1728 bp	0.5	57.1
orf382_p3	TACACGCCTTGAAGGACTTG	GCCGATATTAGCGCAATCCT	50.0	50.0	57.8	58.3	1429 bp	0.5	56.5
orf382_p4	TGAAGGACTTGAACCCTCGA	GCCGATATTAGCGCAATCCT	50.0	50.0	58.3	58.3	1420 bp	0.5	55.5
orf382_p5	AGGACTTGAACCCTCGACAT	GCCGATATTAGCGCAATCCT	50.0	50.0	58.4	58.3	1417 bp	0.5	55.0