Hotspot

ilvB

Genome · 55768-57510 · 1743 bp

Back to Membranoptera

Pi 0.0155

MarkerSeek score 52.50

Primer available yes

Label ilvB

Feature Metadata

label_name: ilvB
feature_type: gene
region: Genome
length_bp: 1743
variable_sites: 27
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvB_p1	TTCGCCTACGTTGGTTCAAA	AAGGTCCTCCTCCATGAACT	45.0	50.0	58.0	57.6	2437 bp	1.0	78.0
ilvB_p2	CCAACCCGGCCCAATAAATA	AAGGTCCTCCTCCATGAACT	50.0	50.0	57.9	57.6	2416 bp	1.0	77.9
ilvB_p3	AATTAGAAAGGGTCGGTGCC	AAGGTCCTCCTCCATGAACT	50.0	50.0	57.9	57.6	2494 bp	1.0	77.4
ilvB_p4	TCGCCTACGTTGGTTCAAAT	AAGGTCCTCCTCCATGAACT	45.0	50.0	57.8	57.6	2436 bp	1.0	77.1
ilvB_p5	ACTGTAAATCCGCTGGCTTC	AAGGTCCTCCTCCATGAACT	50.0	50.0	58.3	57.6	2454 bp	1.0	76.3