Hotspot

ycf63-trnV-UAC

Genome · 174402-174486 · 85 bp

Back to Membranoptera

Pi 0.0353

MarkerSeek score 68.70

Primer available yes

Label ycf63-trnV-UAC

Feature Metadata

label_name: ycf63-trnV-UAC
feature_type: igs
region: Genome
length_bp: 85
variable_sites: 3
pi: 0.0353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf63-trnV-UAC_p1	ACGATTTGTTCTAATACAACAGT	GATTGGGCGGTACTGGATTT	30.4	50.0	54.3	57.9	270 bp	1.0	40.9
ycf63-trnV-UAC_p2	GCACAATAATACGATTTGTTCT	GATTGGGCGGTACTGGATTT	31.8	50.0	53.2	57.9	280 bp	1.0	40.9
ycf63-trnV-UAC_p3	ACGATTTGTTCTAATACAACAGT	AGTTTGATTGGGCGGTACTG	30.4	50.0	54.3	58.2	275 bp	1.0	40.9
ycf63-trnV-UAC_p4	ACGATTTGTTCTAATACAACAGT	GCGGTACTGGATTTGAACCA	30.4	50.0	54.3	58.2	264 bp	1.0	40.9
ycf63-trnV-UAC_p5	ACGATTTGTTCTAATACAACAGT	ATTGGGCGGTACTGGATTTG	30.4	50.0	54.3	58.2	269 bp	1.0	40.9