Hotspot

ycf22-psaC

Genome · 156718-157662 · 945 bp

Back to Membranoptera

Pi 0.0201

MarkerSeek score 67.20

Primer available yes

Label ycf22-psaC

Feature Metadata

label_name: ycf22-psaC
feature_type: igs
region: Genome
length_bp: 945
variable_sites: 19
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf22-psaC_p1	AATCCTGTTTGATTTGTTTCA	GAAACAGCATGCCCTACTGA	28.6	50.0	52.1	57.9	1124-1127 bp	1.0	41.4
ycf22-psaC_p2	ATCCTGTTTGATTTGTTTCAA	GAAACAGCATGCCCTACTGA	28.6	50.0	52.1	57.9	1123-1126 bp	1.0	41.4
ycf22-psaC_p3	TCCTGTTTGATTTGTTTCAAT	GAAACAGCATGCCCTACTGA	28.6	50.0	52.1	57.9	1122-1125 bp	1.0	41.4
ycf22-psaC_p4	TCCTGTTTGATTTGTTTCAAT	GTGAAACAGCATGCCCTACT	28.6	50.0	52.1	58.2	1124-1127 bp	1.0	41.4
ycf22-psaC_p5	ATCCTGTTTGATTTGTTTCAA	GTGAAACAGCATGCCCTACT	28.6	50.0	52.1	58.2	1125-1128 bp	1.0	41.3