Hotspot

psaL-trnT-UGU

Genome · 139798-139857 · 60 bp

Back to Membranoptera

Pi 0.0833

MarkerSeek score 76.30

Primer available yes

Label psaL-trnT-UGU

Feature Metadata

label_name: psaL-trnT-UGU
feature_type: igs
region: Genome
length_bp: 60
variable_sites: 5
pi: 0.0833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaL-trnT-UGU_p1	GGTGCAATAGGAGGTTCTGG	TGGGTTCAAGTCCCCTTACT	55.0	50.0	58.0	57.9	162 bp	1.0	81.1
psaL-trnT-UGU_p2	GGTGCAATAGGAGGTTCTGG	GGTTATGGGTTCAAGTCCCC	55.0	55.0	58.0	57.9	167 bp	1.0	80.9
psaL-trnT-UGU_p3	GGTGCAATAGGAGGTTCTGG	TCAGCAGGTTATGGGTTCAAG	55.0	47.6	58.0	57.9	173 bp	1.0	73.4
psaL-trnT-UGU_p4	GGTGCAATAGGAGGTTCTGG	AGCAGGTTATGGGTTCAAGTC	55.0	47.6	58.0	57.9	171 bp	1.0	73.4
psaL-trnT-UGU_p5	GGTGCAATAGGAGGTTCTGG	TGGTAGAGCAGCTGATTTGT	55.0	45.0	58.0	56.8	195 bp	1.0	72.4