Hotspot

cemA-trnG-UCC

Genome · 136329-138311 · 1983 bp

Back to Membranoptera

Pi 0.0141

MarkerSeek score 58.90

Primer available yes

Label cemA-trnG-UCC

Feature Metadata

label_name: cemA-trnG-UCC
feature_type: igs
region: Genome
length_bp: 1983
variable_sites: 28
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-trnG-UCC_p1	CACCTTCTTCTGTAGCGACA	TTCCAAGCTAATGATGCGGG	50.0	50.0	57.3	58.3	2064 bp	1.0	72.8
cemA-trnG-UCC_p2	CACCTTCTTCTGTAGCGACA	GCTAATGATGCGGGTTCGAT	50.0	50.0	57.3	58.5	2058 bp	1.0	71.4
cemA-trnG-UCC_p3	CACCTTCTTCTGTAGCGACA	CCAAGCTAATGATGCGGGTT	50.0	50.0	57.3	58.6	2062 bp	1.0	70.5
cemA-trnG-UCC_p4	TCACCTTCTTCTGTAGCGAC	TTCCAAGCTAATGATGCGGG	50.0	50.0	57.0	58.3	2065 bp	1.0	70.5
cemA-trnG-UCC_p5	CACCTTCTTCTGTAGCGACA	AATGATGCGGGTTCGATTCC	50.0	50.0	57.3	58.7	2055 bp	1.0	69.8