Hotspot

orf621-cemA

Genome · 134561-135491 · 931 bp

Back to Membranoptera

Pi 0.0301
MarkerSeek score 66.60
Primer available yes
Label orf621-cemA

Feature Metadata

label_name
orf621-cemA
feature_type
igs
region
Genome
length_bp
931
variable_sites
28
pi
0.0301
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf621-cemA_p1 ATTGCTCCAGGTAGAAGACA TGTTTGGTAACGAGCAACCT 45.0 45.0 55.9 57.7 1713-1751 bp 1.0 61.9
orf621-cemA_p2 TTGCTCCAGGTAGAAGACAT TGTTTGGTAACGAGCAACCT 45.0 45.0 55.9 57.7 1712-1750 bp 1.0 61.9
orf621-cemA_p3 ATTGCTCCAGGTAGAAGACAT TGTTTGGTAACGAGCAACCT 42.9 45.0 56.4 57.7 1713-1751 bp 1.0 58.4
orf621-cemA_p4 GTATTGCTCCAGGTAGAAGACA TGTTTGGTAACGAGCAACCT 45.5 45.0 57.3 57.7 1715-1753 bp 1.0 57.2
orf621-cemA_p5 TTGCTCCAGGTAGAAGACAT CGCTAATGTTTGGTAACGAGC 45.0 47.6 55.9 58.0 1718-1756 bp 1.0 56.3