Hotspot

ycf36-trnM-CAU

Genome · 94543-94575 · 33 bp

Back to Membranoptera

Pi 0.0606
MarkerSeek score 74.00
Primer available yes
Label ycf36-trnM-CAU

Feature Metadata

label_name
ycf36-trnM-CAU
feature_type
igs
region
Genome
length_bp
33
variable_sites
2
pi
0.0606
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf36-trnM-CAU_p1 CCTTTCCTGAATAGAATTAAATATACA TACCAGACTGCTCTATCCCG 25.9 55.0 53.7 58.0 139 bp 1.0 41.2
ycf36-trnM-CAU_p2 ACCTTTCCTGAATAGAATTAAATATAC TACCAGACTGCTCTATCCCG 25.9 55.0 53.7 58.0 140 bp 1.0 41.1
ycf36-trnM-CAU_p3 CCTTTCCTGAATAGAATTAAATATACA TTGAACCATTGACCTTCGGG 25.9 50.0 53.7 58.1 176 bp 1.0 40.9
ycf36-trnM-CAU_p4 ACCTTTCCTGAATAGAATTAAATATAC TTGAACCATTGACCTTCGGG 25.9 50.0 53.7 58.1 177 bp 1.0 40.9
ycf36-trnM-CAU_p5 CCTTTCCTGAATAGAATTAAATATACA GCGGGAAATGGATTTGAACC 25.9 50.0 53.7 57.7 189 bp 1.0 40.8