Hotspot

trnD-GUC-ftsH

Genome · 59142-59161 · 20 bp

Back to Membranoptera

Pi 0.05

MarkerSeek score 70.70

Primer available yes

Label trnD-GUC-ftsH

Feature Metadata

label_name: trnD-GUC-ftsH
feature_type: igs
region: Genome
length_bp: 20
variable_sites: 1
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-ftsH_p1	AAGTTGCGGGTTCGAATCC	AGCCAATATACTACAATACCAA	52.6	31.8	58.4	52.4	83 bp	1.0	41.0
trnD-GUC-ftsH_p2	TCAATTGGTTAGAGCACCGC	AGCCAATATACTACAATACCAA	50.0	31.8	58.6	52.4	116 bp	1.0	40.7
trnD-GUC-ftsH_p3	TCAATTGGTTAGAGCACCGC	AGCCAATATACTACAATACCAAT	50.0	30.4	58.6	53.0	116 bp	1.0	40.7
trnD-GUC-ftsH_p4	TCAATTGGTTAGAGCACCGC	AGCCAATATACTACAATACCAATT	50.0	29.2	58.6	53.8	116 bp	1.0	40.7
trnD-GUC-ftsH_p5	AATTGGTTAGAGCACCGCC	AGCCAATATACTACAATACCAA	52.6	31.8	58.1	52.4	114 bp	1.0	40.7