Hotspot

orf238-carA

Genome · 15338-15373 · 36 bp

Back to Membranoptera

Pi 0.0556

MarkerSeek score 68.50

Primer available yes

Label orf238-carA

Feature Metadata

label_name: orf238-carA
feature_type: igs
region: Genome
length_bp: 36
variable_sites: 2
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf238-carA_p1	GCGCCTAAAGGACATTTACC	AGTCCTGGACCTCATGACTC	50.0	55.0	56.9	58.1	204 bp	1.0	71.0
orf238-carA_p2	GCGCCTAAAGGACATTTACC	GTCCTGGACCTCATGACTCT	50.0	55.0	56.9	58.1	203 bp	1.0	71.0
orf238-carA_p3	GCGCCTAAAGGACATTTACC	CCTGGACCTCATGACTCTGA	50.0	55.0	56.9	58.1	201 bp	1.0	71.0
orf238-carA_p4	GCGCCTAAAGGACATTTACC	TCCTGGACCTCATGACTCTG	50.0	55.0	56.9	58.1	202 bp	1.0	71.0
orf238-carA_p5	GCGCCTAAAGGACATTTACC	CATCCAGAAGCAAGTCCTGG	50.0	55.0	56.9	58.3	216 bp	1.0	70.1