Hotspot

ycf35-psbA

Genome · 6477-7306 · 830 bp

Back to Membranoptera

Pi 0.0326

MarkerSeek score 68.90

Primer available yes

Label ycf35-psbA

Feature Metadata

label_name: ycf35-psbA
feature_type: igs
region: Genome
length_bp: 830
variable_sites: 27
pi: 0.0326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35-psbA_p1	GTTTTAATTTTGCTAAAATGTGAC	AAAAACGTTCCCACAGGCTT	25.0	45.0	52.5	58.5	907-908 bp	1.0	42.5
ycf35-psbA_p2	GTTTTAATTTTGCTAAAATGTGAC	AAAGGCGGTTTTCAGTGCTA	25.0	45.0	52.5	58.0	940-941 bp	1.0	42.4
ycf35-psbA_p3	GTTTTAATTTTGCTAAAATGTGAC	TAAAGGCGGTTTTCAGTGCT	25.0	45.0	52.5	58.0	941-942 bp	1.0	42.4
ycf35-psbA_p4	GTTTTAATTTTGCTAAAATGTGAC	GGCGGTTTTCAGTGCTAGTA	25.0	50.0	52.5	57.9	937-938 bp	1.0	42.4
ycf35-psbA_p5	GTTTTAATTTTGCTAAAATGTGAC	AAGGCGGTTTTCAGTGCTAG	25.0	50.0	52.5	58.5	939-940 bp	1.0	42.4