Hotspot

trpG-ccs1

Genome · 2682-3544 · 863 bp

Back to Membranoptera

Pi 0.0384

MarkerSeek score 68.40

Primer available yes

Label trpG-ccs1

Feature Metadata

label_name: trpG-ccs1
feature_type: igs
region: Genome
length_bp: 863
variable_sites: 33
pi: 0.0384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpG-ccs1_p1	AGGACAACCTAGCTCACCAA	TCTGGATCTACTAATCGAGCT	50.0	42.9	58.3	55.1	1634-1637 bp	1.0	49.1
trpG-ccs1_p2	AGGACAACCTAGCTCACCAA	TTCTGGATCTACTAATCGAGCT	50.0	40.9	58.3	55.9	1635-1638 bp	1.0	47.4
trpG-ccs1_p3	AGGACAACCTAGCTCACCAA	TTTCTGGATCTACTAATCGAGCT	50.0	39.1	58.3	56.6	1636-1639 bp	1.0	45.1
trpG-ccs1_p4	GGACAACCTAGCTCACCAAT	TCTGGATCTACTAATCGAGCT	50.0	42.9	57.2	55.1	1633-1636 bp	1.0	45.0
trpG-ccs1_p5	AGGACAACCTAGCTCACCAA	TCTGGATCTACTAATCGAGC	50.0	45.0	58.3	53.5	1634-1637 bp	1.0	44.2