Hotspot

rpoA

LSC · 79197-80204 · 1008 bp

Back to Melodinus

Pi 0.000992

MarkerSeek score 59.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 1
pi: 0.000992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACGATTCAAAATAGATTTTCATCT	AAGACGTGCGTAGAAATGCA	25.0	45.0	53.1	58.2	1161 bp	1.0	40.1
rpoA_p2	ACGATTCAAAATAGATTTTCATCT	AATGGCTGTAGACCTCCGAA	25.0	50.0	53.1	58.4	1184 bp	1.0	40.1
rpoA_p3	ACGATTCAAAATAGATTTTCATCT	ATGGCTGTAGACCTCCGAAA	25.0	50.0	53.1	58.4	1183 bp	1.0	40.1
rpoA_p4	ACGATTCAAAATAGATTTTCATCT	TAATGGCTGTAGACCTCCGA	25.0	50.0	53.1	57.6	1185 bp	1.0	40.1
rpoA_p5	ACGATTCAAAATAGATTTTCATCT	GGCTGTAGACCTCCGAAAAA	25.0	50.0	53.1	57.5	1181 bp	1.0	40.1