Hotspot

ndhF

SSC · 111274-113508 · 2235 bp

Back to Melodinus

Pi 0.000895

MarkerSeek score 44.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 2
pi: 0.000895
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGTTAAACATTAACATTAAGCA	GAGGTAAGATCCGGTCAATCG	26.1	52.4	52.6	58.0	2320 bp	0.5	20.0
ndhF_p2	AGGTTAAACATTAACATTAAGCA	AGGTAAGATCCGGTCAATCG	26.1	50.0	52.6	56.8	2319 bp	0.5	20.0
ndhF_p3	AGGTTAAACATTAACATTAAGCA	GAAAGAGGTAAGATCCGGTCA	26.1	47.6	52.6	56.8	2324 bp	0.5	20.0
ndhF_p4	AGGTTAAACATTAACATTAAGCA	TGAAAGAGGTAAGATCCGGTC	26.1	47.6	52.6	56.8	2325 bp	0.5	20.0
ndhF_p5	AGGTTAAACATTAACATTAAGCA	AGAGGTAAGATCCGGTCAATC	26.1	47.6	52.6	56.6	2321 bp	0.5	20.0