Hotspot

atpF

LSC · 11614-12872 · 1259 bp

Back to Melodinus

Pi 0.00318

MarkerSeek score 60.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1259
variable_sites: 4
pi: 0.00318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCGTCAGCTCGAATGGTT	GAGGGGCGAAGTGATACAAA	45.0	50.0	57.8	57.6	1417-1418 bp	1.0	75.8
atpF_p2	TTTCGTCAGCTCGAATGGTT	AAGAGGGGCGAAGTGATACA	45.0	50.0	57.8	58.4	1419-1420 bp	1.0	75.5
atpF_p3	TTTCGTCAGCTCGAATGGTT	AGAGGGGCGAAGTGATACAA	45.0	50.0	57.8	58.4	1418-1419 bp	1.0	75.5
atpF_p4	TCGTCAGCTCGAATGGTTAC	GAGGGGCGAAGTGATACAAA	50.0	50.0	57.7	57.6	1415-1416 bp	1.0	74.9
atpF_p5	TCGTCAGCTCGAATGGTTAC	AGAGGGGCGAAGTGATACAA	50.0	50.0	57.7	58.4	1416-1417 bp	1.0	74.6