Hotspot

rpl32-trnL-UAG

SSC · 114409-115355 · 947 bp

Back to Melodinus

Pi 0.00106

MarkerSeek score 59.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 947
variable_sites: 1
pi: 0.00106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAAGGATATTGGACAGCGT	TTAGAAGATGACATGCCGCC	47.6	50.0	58.1	58.0	1147-1152 bp	1.0	71.1
rpl32-trnL-UAG_p2	GGAAAGGATATTGGACAGCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	58.1	57.9	1093-1098 bp	1.0	70.5
rpl32-trnL-UAG_p3	AGGAAAGGATATTGGACAGCG	TTAGAAGATGACATGCCGCC	47.6	50.0	57.8	58.0	1148-1153 bp	1.0	70.2
rpl32-trnL-UAG_p4	GGAAAGGATATTGGACAGCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	58.1	57.8	1086-1091 bp	1.0	70.0
rpl32-trnL-UAG_p5	GGAAAGGATATTGGACAGCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	58.1	57.8	1091-1096 bp	1.0	69.9