Hotspot

ndhF-rpl32

SSC · 113509-114237 · 729 bp

Back to Melodinus

Pi 0

MarkerSeek score 59.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 729
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACGCTGTCCAATATCCTTTCC	50.0	47.6	58.0	58.1	908-912 bp	1.0	71.4
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	ACGCTGTCCAATATCCTTTCC	45.0	47.6	58.0	58.1	906-910 bp	1.0	71.4
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	ACGCTGTCCAATATCCTTTCC	45.0	47.6	58.0	58.1	907-911 bp	1.0	71.4
ndhF-rpl32_p4	TCGGAAAAAGAAGAAGCCCC	ACGCTGTCCAATATCCTTTCC	50.0	47.6	58.1	58.1	901-905 bp	1.0	71.0
ndhF-rpl32_p5	GGTATGAGCCACCCGTATTG	ACGCTGTCCAATATCCTTTCC	55.0	47.6	58.1	58.1	845-849 bp	1.0	70.8