Hotspot

trnN-GUU-ndhF

IRb · 109832-111273 · 1442 bp

Back to Melodinus

Pi 0.00139

MarkerSeek score 51.80

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1442
variable_sites: 2
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	CGGGCGCATCTCTTCTTATC	50.0	55.0	58.2	58.6	1583-1589 bp	1.0	74.5
trnN-GUU-ndhF_p2	CCCCAAGTAGGATTCGAACC	CGGGCGCATCTCTTCTTATC	55.0	55.0	57.7	58.6	1591-1597 bp	1.0	73.2
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	GGGCGCATCTCTTCTTATCT	50.0	50.0	58.2	57.2	1582-1588 bp	1.0	72.5
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	ATCAAATATGTAGGGGGCGG	50.0	50.0	58.2	57.1	1600-1606 bp	1.0	72.1
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	GGGCGCATCTCTTCTTATCT	55.0	50.0	57.7	57.2	1590-1596 bp	1.0	71.1