Hotspot

psbE-petL

LSC · 66085-67266 · 1182 bp

Back to Melodinus

Pi 0.000855

MarkerSeek score 60.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1182
variable_sites: 1
pi: 0.000855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1317-1329 bp	1.0	71.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1316-1328 bp	1.0	71.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.8	56.9	1240-1252 bp	1.0	70.4
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1239-1251 bp	1.0	70.4
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.2	56.9	1325-1337 bp	1.0	70.3