Hotspot

ycf4-cemA

LSC · 61714-62401 · 688 bp

Back to Melodinus

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAGAAAACCACCAAGGCA	45.0	50.0	58.1	58.0	793 bp	1.0	79.2
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGAGAAAACCACCAAGGCA	45.0	50.0	57.8	58.0	807 bp	1.0	78.6
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGAGAAAACCACCAAGGCA	45.0	50.0	57.8	58.0	805 bp	1.0	78.2
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AGAGAGAAAACCACCAAGGC	45.0	50.0	58.1	57.7	794 bp	1.0	77.1
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	AGAGAGAAAACCACCAAGGC	45.0	50.0	57.8	57.7	808 bp	1.0	76.5