Hotspot

trnT-GGU-psbD

LSC · 32501-33692 · 1192 bp

Back to Melodinus

Pi 0.000839

MarkerSeek score 59.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1192
variable_sites: 1
pi: 0.000839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1348 bp	1.0	79.2
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1366 bp	1.0	78.3
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1311 bp	1.0	77.8
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1356 bp	1.0	77.3
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1329 bp	1.0	76.9