Hotspot

rps16-trnQ-UUG

LSC · 6143-7353 · 1211 bp

Back to Melodinus

Pi 0.00248
MarkerSeek score 60.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1211
variable_sites
3
pi
0.00248
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAAATCAAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1342 bp 1.0 79.0
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAAATCAAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1353 bp 1.0 78.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1284 bp 1.0 78.5
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1295 bp 1.0 77.9
rps16-trnQ-UUG_p5 ATCATGTCCTTCAAGTCGCA GAAATCAAAATGGGGCGTGG 45.0 50.0 57.2 58.0 1354 bp 1.0 74.0