Hotspot

rpoC2

LSC · 26826-31385 · 4560 bp

Back to Melocalamus

Pi 0.000292

MarkerSeek score 53.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4560
variable_sites: 2
pi: 0.000292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCGAATCCTACTCAGCCGAA	AAAACTCTGTTGTTCCGCGT	50.0	45.0	58.2	58.6	277 bp	1.0	73.4
rpoC2_p2	CCTACTCAGCCGAAAAAGGG	AAAACTCTGTTGTTCCGCGT	55.0	45.0	58.3	58.6	271 bp	1.0	73.2
rpoC2_p3	CTACTCAGCCGAAAAAGGGG	AAAACTCTGTTGTTCCGCGT	55.0	45.0	58.3	58.6	270 bp	1.0	73.2
rpoC2_p4	TTCGAATCACTGACTCAGGC	AAAACTCTGTTGTTCCGCGT	50.0	45.0	57.6	58.6	324 bp	1.0	72.3
rpoC2_p5	GGCCCATTGTCGAATCCTAC	AAAACTCTGTTGTTCCGCGT	55.0	45.0	58.4	58.6	307 bp	1.0	72.0