Hotspot

ndhB

IRa · 131494-133738 · 2245 bp

Back to Melocalamus

Pi 0

MarkerSeek score 31.40

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRa
length_bp: 2245
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ACAATCGGGACTTTTCGGAG	AAGTTCATAACCCTAGCGCC	50.0	50.0	57.9	57.7	2392 bp	1.0	76.6
ndhB_p2	ACAATCGGGACTTTTCGGAG	TTTGGGCGGAACAGATCTAC	50.0	50.0	57.9	57.6	2363 bp	1.0	76.1
ndhB_p3	ACAATCGGGACTTTTCGGAG	TTGGGCGGAACAGATCTACT	50.0	50.0	57.9	58.4	2362 bp	1.0	75.8
ndhB_p4	ACAATCGGGACTTTTCGGAG	TGGGCGGAACAGATCTACTA	50.0	50.0	57.9	57.6	2361 bp	1.0	75.7
ndhB_p5	ACAATCGGGACTTTTCGGAG	CATAACCCTAGCGCCCATTC	50.0	55.0	57.9	58.5	2387 bp	1.0	75.4