Hotspot

ndhB-trnL-CAA

LSC · 133739-134303 · 565 bp

Back to Melocalamus

Pi 0.0142

MarkerSeek score 67.00

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 12
pi: 0.0142
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	625 bp	1.0	81.0
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	623 bp	1.0	81.0
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	620 bp	1.0	79.5
ndhB-trnL-CAA_p4	CCCCATTCTTGCAATTGCTC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.7	58.0	634 bp	1.0	79.0
ndhB-trnL-CAA_p5	CCCCATTCTTGCAATTGCTC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.7	58.0	632 bp	1.0	79.0