Hotspot

ndhC-trnV-UAC

LSC · 52116-53040 · 925 bp

Back to Melocalamus

Pi 0.000742

MarkerSeek score 61.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 1
pi: 0.000742
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1025-1051 bp	1.0	81.2
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	1026-1052 bp	1.0	79.4
ndhC-trnV-UAC_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1024-1050 bp	1.0	78.8
ndhC-trnV-UAC_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1027-1053 bp	1.0	77.6
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	1025-1051 bp	1.0	76.9