Hotspot

atpH-atpF

LSC · 34445-34900 · 456 bp

Back to Melocalamus

Pi 0

MarkerSeek score 47.30

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AGTTGTGGCACTAGCACTTT	GGATGGCCAGTGAGCTAAAA	45.0	50.0	57.7	57.9	531-547 bp	1.0	78.5
atpH-atpF_p2	AGTTGTGGCACTAGCACTTT	ATTAAGCCCGAAACTCCCAG	45.0	50.0	57.7	57.6	552-568 bp	1.0	76.1
atpH-atpF_p3	CTAGTTGTGGCACTAGCACT	GGATGGCCAGTGAGCTAAAA	50.0	50.0	57.2	57.9	533-549 bp	1.0	75.3
atpH-atpF_p4	CTAGTTGTGGCACTAGCACTT	GGATGGCCAGTGAGCTAAAA	47.6	50.0	58.0	57.9	533-549 bp	1.0	73.0
atpH-atpF_p5	CTAGTTGTGGCACTAGCACT	ATTAAGCCCGAAACTCCCAG	50.0	50.0	57.2	57.6	554-570 bp	1.0	72.9