Hotspot

ycf1

SSC · 121778-127312 · 5535 bp

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Pi 0.00403

MarkerSeek score 47.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5535
variable_sites: 33
pi: 0.00403
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTCGTTTCATAGCCCGG	ATTGCATCTCCGAGTTGGAC	50.0	50.0	58.1	58.0	677 bp	1.0	80.5
ycf1_p2	CTATACAACGCTGGCGAACT	ATTGCATCTCCGAGTTGGAC	50.0	50.0	58.1	58.0	784 bp	1.0	80.2
ycf1_p3	TATACAACGCTGGCGAACTC	ATTGCATCTCCGAGTTGGAC	50.0	50.0	58.1	58.0	783 bp	1.0	80.1
ycf1_p4	AACGCTGGCGAACTCTATTT	ATTGCATCTCCGAGTTGGAC	45.0	50.0	57.9	58.0	778 bp	1.0	80.0
ycf1_p5	TTAGCTATACAACGCTGGCG	ATTGCATCTCCGAGTTGGAC	50.0	50.0	58.2	58.0	788 bp	1.0	79.6