Hotspot

rpoA

LSC · 76912-77919 · 1008 bp

Back to Melissa

Pi 0.000661

MarkerSeek score 32.60

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 1
pi: 0.000661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TTGGTTGGGTATTGGTGCAA	GACTCACTAAAGGGCTCAGC	45.0	55.0	57.9	58.0	1809-1831 bp	1.0	80.8
rpoA_p2	TTTGGTTGGGTATTGGTGCA	GACTCACTAAAGGGCTCAGC	45.0	55.0	57.9	58.0	1810-1832 bp	1.0	80.8
rpoA_p3	CTTCGGACAGTGCCCAATAA	GACTCACTAAAGGGCTCAGC	50.0	55.0	57.9	58.0	1970-1992 bp	1.0	80.7
rpoA_p4	CAAAATCCATTTCGCCGTCC	GACTCACTAAAGGGCTCAGC	50.0	55.0	58.1	58.0	1893 bp	0.666667	65.9
rpoA_p5	CCAAAATCCATTTCGCCGTC	GACTCACTAAAGGGCTCAGC	50.0	55.0	58.1	58.0	1894 bp	0.666667	65.9