Hotspot

petB

LSC · 73978-75357 · 1380 bp

Back to Melissa

Pi 0.0101
MarkerSeek score 58.20
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1380
variable_sites
21
pi
0.0101
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TTGGTAGTTCGATCGTGGAA TCCTATCATTCCTCCTCTAAGTGA 45.0 41.7 56.6 57.7 1469-1487 bp 1.0 42.3
petB_p2 TTTGGTAGTTCGATCGTGGA TCCTATCATTCCTCCTCTAAGTGA 45.0 41.7 56.6 57.7 1470-1488 bp 1.0 42.3
petB_p3 TGGTAGTTCGATCGTGGAAT TCCTATCATTCCTCCTCTAAGTGA 45.0 41.7 56.4 57.7 1468-1486 bp 1.0 42.3
petB_p4 TTTGGTAGTTCGATCGTGGAA TCCTATCATTCCTCCTCTAAGTGA 42.9 41.7 57.3 57.7 1470-1488 bp 1.0 42.3
petB_p5 TTTTGGTAGTTCGATCGTGGA TCCTATCATTCCTCCTCTAAGTGA 42.9 41.7 57.3 57.7 1471-1489 bp 1.0 42.3