Hotspot

ndhH

SSC · 119824-121059 · 1236 bp

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Pi 0.00162

MarkerSeek score 59.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1236
variable_sites: 3
pi: 0.00162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GACAAGCATCCATATGATCCCA	ACCATTTGATGAGCTTAGTCGT	45.5	40.9	58.0	57.8	1362 bp	1.0	46.3
ndhH_p2	AGGGACAAGCATCCATATGA	ACCATTTGATGAGCTTAGTCGT	45.0	40.9	56.0	57.8	1365 bp	1.0	46.3
ndhH_p3	ACAAGCATCCATATGATCCCA	ACCATTTGATGAGCTTAGTCGT	42.9	40.9	56.8	57.8	1361 bp	1.0	45.1
ndhH_p4	AGGGACAAGCATCCATATGATC	ACCATTTGATGAGCTTAGTCGT	45.5	40.9	57.7	57.8	1365 bp	1.0	44.1
ndhH_p5	TGAAAATAGGGACAAGCATCCA	ACCATTTGATGAGCTTAGTCGT	40.9	40.9	57.7	57.8	1372 bp	1.0	44.0