Hotspot

ndhG-ndhI

SSC · 116753-117123 · 371 bp

Back to Melissa

Pi 0.00898

MarkerSeek score 59.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 371
variable_sites: 5
pi: 0.00898
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAATGAAAAGGCCGAAT	TCAAATTGCTTTAGGCCGGT	45.0	45.0	57.8	58.1	585-589 bp	1.0	78.2
ndhG-ndhI_p2	TCCCAATGAAAAGGCCGAAT	ATTGCTTTAGGCCGGTTACC	45.0	50.0	57.8	58.2	581-585 bp	1.0	76.9
ndhG-ndhI_p3	TCCCAATGAAAAGGCCGAAT	CAAATTGCTTTAGGCCGGTT	45.0	45.0	57.8	57.3	584-588 bp	1.0	72.9
ndhG-ndhI_p4	TCCCAATGAAAAGGCCGAATA	TCAAATTGCTTTAGGCCGGT	42.9	45.0	57.6	58.1	585-589 bp	1.0	69.0
ndhG-ndhI_p5	TCCCAATGAAAAGGCCGAAT	TTGCTTTAGGCCGGTTACC	45.0	52.6	57.8	57.7	580-584 bp	1.0	68.8