Hotspot

rpl16-rps3

LSC · 81438-81579 · 142 bp

Back to Melissa

Pi 0.0141

MarkerSeek score 61.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 3
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CTATCGGGTCCGGTCTTTTG	ATCTATGGGGTCTTGGGCAT	55.0	50.0	58.1	58.2	263 bp	1.0	79.1
rpl16-rps3_p2	CTATCGGGTCCGGTCTTTTG	CCCTACAAACCATTCGAGCT	55.0	50.0	58.1	57.6	316 bp	1.0	77.0
rpl16-rps3_p3	CTATCGGGTCCGGTCTTTTG	GGGGTCTTGGGCATCAAAAT	55.0	50.0	58.1	58.4	257 bp	1.0	77.0
rpl16-rps3_p4	TTTTCACACTATCGGGTCCG	ATCTATGGGGTCTTGGGCAT	50.0	50.0	57.6	58.2	271 bp	1.0	76.6
rpl16-rps3_p5	AAACCAACGAGTCACACACT	ATCTATGGGGTCTTGGGCAT	45.0	50.0	57.6	58.2	225 bp	1.0	76.6