Hotspot

psaJ-rpl33

LSC · 66387-66835 · 449 bp

Back to Melissa

Pi 0.00742

MarkerSeek score 60.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 5
pi: 0.00742
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	TCCAAAATGACCGTTACCCG	45.0	50.0	58.1	58.2	522 bp	1.0	78.6
psaJ-rpl33_p2	TGCGTTGACATTCCCCTTTT	TCCAAAATGACCGTTACCCG	45.0	50.0	58.3	58.2	521 bp	1.0	76.8
psaJ-rpl33_p3	CTATGGTTCGGGGCTTTAGC	TCCAAAATGACCGTTACCCG	55.0	50.0	58.4	58.2	577 bp	1.0	75.9
psaJ-rpl33_p4	GCTATGGTTCGGGGCTTTAG	TCCAAAATGACCGTTACCCG	55.0	50.0	58.4	58.2	578 bp	1.0	75.9
psaJ-rpl33_p5	ATGCGTTGACATTCCCCTTT	TTACCCGCGCATCTTTCTTC	45.0	50.0	58.1	58.6	509 bp	1.0	75.1